Primary Cells Versus Cell lines

Primary cells: Primary cells are separated directly from human or animal tissue. They are then put in an artificial environment in glass or plastic containers with specific media containing essential nutrients and growth hormones to aid proliferation. Despite the fact that primary cells have a finite lifespan, they have a lot of benefits over cell lines. Researchers have had the ability to investigate donors as well as cells when they do primary cell culture.

Cell lines: There are two types of cell lines: finite and continuous. By genetic mutations or artificial alterations, an immortalized or continuous cell line has gained the potential to multiply continuously. After being sub-cultured for 20–80 passages, a limited cell line senesces. For ease of usage, cell lines are used since they are simple to handle and widely available. However, because they have acquired the genuine features of the original tissue from which they have been separated, they are less regarded as a physiologically relevant choice.